nhdf fibroblast basal medium  (PromoCell)

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Expression and activity of IL-17 receptor subunits in human cutaneous cells as targets for anti-IL-17 therapeutic antibodies.

doi: 10.1016/j.biopha.2021.112569

Figure Lengend Snippet: Fig. 2. Western blot analysis in NHEK, ESC, NHDF, MSC, HDMEC, and LEC A: basal expression levels of IL-17 receptor subunits (3 different donors) B: activation of MAP-MEK-ERK/p38 after stimulation by different ligands of IL-17.

Article Snippet: Cell type-specific media and supplements were used for cell culture (NHEK and ESC: Keratinocyte-SFM Gibco (Fisher, Schwerte, Germany) with Supplement: Human Recombinant Epidermal Growth Factor and Bovine Pituitary Extract; NHDF: Fibroblast Basal Medium (Promocell, Heidelberg, Germany) with supplement BFGF/Insulin; MSC: Mesenchymal Stem Cell Basal Medium (Lonza Ltd, Basel, Switzerland) with Supplement: MCGS/L-Glutamine/GA-1000; HDMEC and LEC: Endothelial cell Growth Medium MV2 (Promocell, Heidelberg, Germany) with Supplement: VEGF/MixMV2).

Techniques: Western Blot, Expressing, Activation Assay

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Expression and activity of IL-17 receptor subunits in human cutaneous cells as targets for anti-IL-17 therapeutic antibodies.

doi: 10.1016/j.biopha.2021.112569

Figure Lengend Snippet: Fig. 1. Expression of the IL-17 receptor subunits in NHEK, ESC, NHDF, MSC, HDMEC, and LEC after stimulation with 100 ng/mL IL-17A. The data represent the gene expression relative to vehicle treated control (dotted line). Bars represent average ± standard deviation of at least three independent experi ments. Student T-test in comparison to control, * p ≤0.05; ** p ≤0.01.

Article Snippet: Cell type-specific media and supplements were used for cell culture (NHEK and ESC: Keratinocyte-SFM Gibco (Fisher, Schwerte, Germany) with Supplement: Human Recombinant Epidermal Growth Factor and Bovine Pituitary Extract; NHDF: Fibroblast Basal Medium (Promocell, Heidelberg, Germany) with supplement BFGF/Insulin; MSC: Mesenchymal Stem Cell Basal Medium (Lonza Ltd, Basel, Switzerland) with Supplement: MCGS/L-Glutamine/GA-1000; HDMEC and LEC: Endothelial cell Growth Medium MV2 (Promocell, Heidelberg, Germany) with Supplement: VEGF/MixMV2).

Techniques: Expressing, Gene Expression, Control, Standard Deviation, Comparison

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Expression and activity of IL-17 receptor subunits in human cutaneous cells as targets for anti-IL-17 therapeutic antibodies.

doi: 10.1016/j.biopha.2021.112569

Figure Lengend Snippet: Fig. 3. Analysis of NF-κB subunit translocation into the nucleus upon addition of IL-17 ligands (IL-17A, IL-17C, IL-17E, IL-17F) in NHEK, NHDF, MSC, HDMEC, and LEC. (left = DAPI, center = α p65, right = merge).

Article Snippet: Cell type-specific media and supplements were used for cell culture (NHEK and ESC: Keratinocyte-SFM Gibco (Fisher, Schwerte, Germany) with Supplement: Human Recombinant Epidermal Growth Factor and Bovine Pituitary Extract; NHDF: Fibroblast Basal Medium (Promocell, Heidelberg, Germany) with supplement BFGF/Insulin; MSC: Mesenchymal Stem Cell Basal Medium (Lonza Ltd, Basel, Switzerland) with Supplement: MCGS/L-Glutamine/GA-1000; HDMEC and LEC: Endothelial cell Growth Medium MV2 (Promocell, Heidelberg, Germany) with Supplement: VEGF/MixMV2).

Techniques: Translocation Assay

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Expression and activity of IL-17 receptor subunits in human cutaneous cells as targets for anti-IL-17 therapeutic antibodies.

doi: 10.1016/j.biopha.2021.112569

Figure Lengend Snippet: Fig. 4. qRT-PCR analysis of cytokine expression levels upon addition of IL-17A, IL-17C, IL-17E and IL-17F in NHEK, NHDF, MSC, HDMEC, and LEC. Heatmap shows the expression level of analyzed genes.

Article Snippet: Cell type-specific media and supplements were used for cell culture (NHEK and ESC: Keratinocyte-SFM Gibco (Fisher, Schwerte, Germany) with Supplement: Human Recombinant Epidermal Growth Factor and Bovine Pituitary Extract; NHDF: Fibroblast Basal Medium (Promocell, Heidelberg, Germany) with supplement BFGF/Insulin; MSC: Mesenchymal Stem Cell Basal Medium (Lonza Ltd, Basel, Switzerland) with Supplement: MCGS/L-Glutamine/GA-1000; HDMEC and LEC: Endothelial cell Growth Medium MV2 (Promocell, Heidelberg, Germany) with Supplement: VEGF/MixMV2).

Techniques: Quantitative RT-PCR, Expressing

Journal: Science and Technology of Advanced Materials

Article Title: Characterization and evaluation of the ability of graphene quantum dots to affect α-synuclein aggregation in synucleinopathy models

doi: 10.1080/14686996.2026.2662693

Figure Lengend Snippet: Viability of NHDF cells exposed to different concentrations of GQDs, as determined by the MTT assay, at 24 h (A), 48 h (B), and 72 h (C). Control cells were assigned 100% viability. The experiments were conducted in quadruplicate, and the data are expressed as median ± interquartile range (IQR). * p < 0.05, ** p < 0.01, *** p < 0.001 by Kruskal – Wallis test with Dunn’s multiple comparison test.

Article Snippet: NHDF cells (PromoCell, C-23210, Heidelberg, Germany) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Sigma, D0819) supplemented with 10% fetal bovine serum (FBS; Sigma, Lot: 0001653683), 1 μg mL −1 penicillin-streptomycin (Sigma, Lot: 0000191002), and 2 mM L-glutamine (Sigma, RNBL6712).

Techniques: MTT Assay, Control, Comparison

Journal: Science and Technology of Advanced Materials

Article Title: Characterization and evaluation of the ability of graphene quantum dots to affect α-synuclein aggregation in synucleinopathy models

doi: 10.1080/14686996.2026.2662693

Figure Lengend Snippet: Ic 5 0 values of GQDs in NHDF cells at 24 h (A), 48 h (B), and 72 h (C) are shown in panels A, B, and C, respectively. Data were analyzed using log-linear regression in GraphPad prism. Individual data points are shown in green (mean ± sd, where applicable). The blue curves represent the best-fit log-linear regression models. Each panel represents an independent dataset or experimental condition analyzed under the same fitting parameters.

Article Snippet: NHDF cells (PromoCell, C-23210, Heidelberg, Germany) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Sigma, D0819) supplemented with 10% fetal bovine serum (FBS; Sigma, Lot: 0001653683), 1 μg mL −1 penicillin-streptomycin (Sigma, Lot: 0000191002), and 2 mM L-glutamine (Sigma, RNBL6712).

Techniques:

Journal: Science and Technology of Advanced Materials

Article Title: Characterization and evaluation of the ability of graphene quantum dots to affect α-synuclein aggregation in synucleinopathy models

doi: 10.1080/14686996.2026.2662693

Figure Lengend Snippet: Expression profiles of DDR-related proteins in NHDF cells following 24 h exposure to GQDs at 90 µg mL −1 . (A) Antibody array layout showing antigen-specific antibody spots; ‘nbs1’ control spots were used for data normalization, and ‘NEG’ spots served as negative controls for baseline signal measurement. (B) Representative images of the original antibody arrays. (C) heat map illustrating the relative expression levels of DDR-related proteins, with color intensity indicating normalized expression values. Data represent four independent experiments ( n = 4). (D) semi-quantitative analysis of DDR-related proteins expression using antibody microarray in NHDF cells treated with GQDs at 90 µg mL −1 for 24 h. Data are expressed as mean ± standard deviation (sd) ( n = 4) relative to control cells. Statistical significance was assessed using one-way ANOVA, followed by Sidak’s multiple comparisons test: ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. control. (e) GQDs induced differential DDR pathway modulation. Pathway–gene interaction heatmap visualized the relationship between key signaling pathways (rows) and DDR-related proteins (columns). Color scale denotes expression changes, with red indicating up-regulation, blue indicating down-regulation.

Article Snippet: NHDF cells (PromoCell, C-23210, Heidelberg, Germany) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Sigma, D0819) supplemented with 10% fetal bovine serum (FBS; Sigma, Lot: 0001653683), 1 μg mL −1 penicillin-streptomycin (Sigma, Lot: 0000191002), and 2 mM L-glutamine (Sigma, RNBL6712).

Techniques: Expressing, Ab Array, Control, Microarray, Standard Deviation, Protein-Protein interactions

Journal: Science and Technology of Advanced Materials

Article Title: Characterization and evaluation of the ability of graphene quantum dots to affect α-synuclein aggregation in synucleinopathy models

doi: 10.1080/14686996.2026.2662693

Figure Lengend Snippet: Expression profiles of cytokine-related factors in NHDF cells following 24 h treatment with GQDs at 90 µg mL −1 . A human cytokine antibody array containing 40 cytokines was used, with ‘pos’ positive control spots applied for data normalization. (A) Each antibody was spotted in quadruplicate in a horizontal layout. (B) Representative fluorescence images of the cytokine antibody arrays. (C) Upregulated cytokines and downregulated cytokines were identified in NHDF cells treated with GQDs (90 µg mL −1 , 24 h). The array detected two significantly upregulated cytokines compared to control cells. Eight cytokines were significantly downregulated, intercellular adhesion molecule, C-X-C motif chemokine 9, interleukins/, and metalloproteinase inhibitors. Statistical analysis was receptors performed using unpaired Student’s t-test. * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.0001 vs. control. (D) Kyoto encyclopedia of genes and genomes (KEGG) showing immune pathway modulation following GQD exposure. Horizontal bar plot illustrating KEGG pathway enrichment based on log2 Fold change (GQDs/control). Negative values indicate pathway suppression and positive values indicate pathway activation following GQD exposure. GQDs markedly suppressed inflammatory and adhesion-related pathways while enhancing Th2-associated (IL-5) immune signaling.

Article Snippet: NHDF cells (PromoCell, C-23210, Heidelberg, Germany) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Sigma, D0819) supplemented with 10% fetal bovine serum (FBS; Sigma, Lot: 0001653683), 1 μg mL −1 penicillin-streptomycin (Sigma, Lot: 0000191002), and 2 mM L-glutamine (Sigma, RNBL6712).

Techniques: Expressing, Ab Array, Positive Control, Fluorescence, Control, Activation Assay